Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification

نویسندگان

  • Linna Liu
  • Hongwei Gao
  • Jiping Li
  • Ying Dong
  • Ning Liu
  • Jiayu Wan
  • Wensen Liu
  • Yucheng Sun
  • Ming Xu
چکیده

The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.

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عنوان ژورنال:
  • International Journal of Molecular Sciences

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2009